Analysis of glucose-6-phosphate translocase and hexose-6-phosphate phosphohydrolase, the two obligatory components of microsomal glucose-6-phosphatase system, in rat liver.
نویسندگان
چکیده
منابع مشابه
Permeability of rat liver microsomal membrane to glucose 6-phosphate.
Light-scattering measurements of osmotically induced changes in the size of rat liver microsomal vesicles pre-equilibrated in a low-osmolality buffer revealed the following. (1) The increase in extravesicular osmolality by addition of glucose 6-phosphate or mannose 6-phosphate (25 mM each) caused a rapid shrinking of microsomal vesicles. After shrinkage, a rapid swelling phase (t1/2 approx. 22 ...
متن کاملDetermination of liver microsomal glucose-6-phosphatase.
A procedure for the determination of liver microsomal glucose-6-phosphatase is described. Homogenization and ultracentrifrigation were used to prepare a precipitate whose character was defined by monitoring the desire enzyme activity which serves as a marker. Activity of the enzyme was determined by means of a sensitive colorimetric reaction for the product, inorganic phosphate. Non-enzymatic h...
متن کاملLiver Microsomal Glucose 6 - Phosphatase , Inorganic Pyrophosphatase , and Pyrophosphate - Glucose
Recently, we (1) described experiments which strongly supported the common identity of glucose 6-phosphatase,’ inorganic pyrophosphatase, pyrophosphate-glucose phosphotransferase, and mannose B-phosphate-glucose phosphotransferase activities present in a preparation obtained by ammonium sulfate fractionation of deoxycholate-dispersed rat liver microsomes. Previously, Segal, Washko, and Lee (2, ...
متن کاملGlucose-6-Phosphate Dehydrogenase
Description Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency is the most common human enzyme deficiency that mainly affects the red blood cells (RBCs). A defect in G6PD enzyme leads to the destruction of premature RBCs causing hemolytic anemia because the body can not compensate the destroyed RBCs. Thus, the affected individuals show jaundice (paleness, yellowing of the skin and whites of th...
متن کاملAccessibility of glucose 6-phosphate: phosphohydrolase to antibody attack in modified microsomal vesicles.
Recent investigations supported the existence of 2 components of the endoplasmic reticulum participating in the process of glucose 6-phosphate hydrolysis: the glucose 6-phosphate-specific transporter that mediates the movement of the substrate from the cytoplasmic membrane surface into the lumen and the unspecific phosphohydrolase on the luminal side of the membrane [ 1,2]. However, this model ...
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ژورنال
عنوان ژورنال: The Tohoku Journal of Experimental Medicine
سال: 1988
ISSN: 0040-8727,1349-3329
DOI: 10.1620/tjem.155.173